Fig. 4

Nkd^WT, Nkd^NN, and Nkd^HHdifferential rescue in Wnt/PCP. (A-E) Nkd^WT can rescue DFC clusters (A-D’) Images of DFCs from a sox17 in situ, zoom in in A’-D’. (A-A’) Normal, clustered DFC, (B-B’) An abnormal example of spread out DFCs, (C-C’) An abnormal example of two clusters, (D-D’) An abnormal example of a loss of the DFC cluster, (E) Nkd^WTRNA restores the DFC cluster in NkdMO+Nkd^WT injected embryos, while Nkd^NN RNA and Nkd^HH RNA fails to rescue the cluster morphology of DFCs in NkdMO injected embryos. Uninjected n=145, NkdMO alone n=90, NkdMO+GAPDH n=58, NkdMO+Nkd^WT n=59, NkdMO+Nkd^NN n=50, NkdMO+Nkd^HH n=74. *=p-value<0.01, **=p-value<0.001, ***=p-value<0.0001, Fisher's exact test. (F-I) Nkd Rescue of Left-Right Patterning. (F-H) Spaw in situs on 19–23 ss (18–20 hpf). (F) Normal left-sided expression. (G) Abnormal bilateral expression. (H) Abnormal right-sided expression. (I) Nkd^WT and Nkd^NN can rescue abnormal spaw expression, while Nkd^HH does not. Uninjected n=167, NkdMO+control n=126, NkdMO+Nkd^WT n=167, NkdMO+Nkd^NN n=105, NkdMO+Nkd^HH n=59. Fisher's exact test. *=p-value<0.01, **=p-value<0.001. See also Figs. S6 and S7.