Fig. 4
NkdWT, NkdNN, and NkdHHdifferential rescue in Wnt/PCP. (A-E) NkdWT can rescue DFC clusters (A-D’) Images of DFCs from a sox17 in situ, zoom in in A’-D’. (A-A’) Normal, clustered DFC, (B-B’) An abnormal example of spread out DFCs, (C-C’) An abnormal example of two clusters, (D-D’) An abnormal example of a loss of the DFC cluster, (E) NkdWTRNA restores the DFC cluster in NkdMO+NkdWT injected embryos, while NkdNN RNA and NkdHH RNA fails to rescue the cluster morphology of DFCs in NkdMO injected embryos. Uninjected n=145, NkdMO alone n=90, NkdMO+GAPDH n=58, NkdMO+NkdWT n=59, NkdMO+NkdNN n=50, NkdMO+NkdHH n=74. *=p-value<0.01, **=p-value<0.001, ***=p-value<0.0001, Fisher's exact test. (F-I) Nkd Rescue of Left-Right Patterning. (F-H) Spaw in situs on 19–23 ss (18–20 hpf). (F) Normal left-sided expression. (G) Abnormal bilateral expression. (H) Abnormal right-sided expression. (I) NkdWT and NkdNN can rescue abnormal spaw expression, while NkdHH does not. Uninjected n=167, NkdMO+control n=126, NkdMO+NkdWT n=167, NkdMO+NkdNN n=105, NkdMO+NkdHH n=59. Fisher's exact test. *=p-value<0.01, **=p-value<0.001. See also Figs. S6 and S7.
Reprinted from Developmental Biology, 434(1), Marsden, A.N., Derry, S.W., Schneider, I., Scott, C.A., Westfall, T.A., Brastrom, L.K., Shea, M., Dawson, D.V., Slusarski, D.C., The Nkd EF-Hand Domain Modulates Divergent Wnt Signaling Outputs in Zebrafish, 63-73, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.