Fig. 3

Nkd^WT, Nkd^NN, and Nkd^HHantagonize Wnt/β-catenin. (A-D) Live images of 28hpf embryos, lateral view, anterior to the left. (A) Wild-type, (B) Wnt8+GAPDH RNAs, (C) Wnt8+Nkd^WT RNAs, and (D) Wnt8+Nkd^NN RNAs. (E) Graph of percentage of embryos without eyes. hpf, hours post fertilization. Uninj n=230, Wnt8 alone n=148, Wnt8+GAPDH n=34, Wnt8+Nkd^WT n=71, Wnt8+Nkd^NN n=44, Wnt8+Nkd^HH n=53. ***=p-value<0.0001, **=p-value<0.001, Fishers Exact Test. (F-G’) Confocal images of DFCs stained with anti- β-catenin. Nkd Antagonizes β-catenin Nuclear Localization in the DFCs. (F and G) Sox17-GFP which marks the DFCs. (F’ and G’) anti-β-catenin staining. (F-F’) Uninjected Sox17. (G-G’) NkdMO injected transgenic Sox17 line. (H) Graph showing percent (%) of embryos with β-catenin and Sox17 positive nuclei. Note that all forms of Nkd were able to rescue morphantsto uninjected levels, and that Nkd^HH was the most robust. Sox17 uninj. n=211, NkdMO alone n=134, NkdMO+GAPDH n=153, NkdMO+Nkd^WT n=70, NkdMO+Nkd^NN n=77, NkdMO+Nkd^HH n=99. n=number of cells. ****=p-value<1×10⁻⁵, ***=p-value<0.0001, Fishers Exact Test. Scale bar 10 µm. Confocal images taken at 63x at 2x zoom. See also Fig. S5.