Fig. S6
- ID
- ZDB-FIG-180410-33
- Publication
- Sawamiphak et al., 2017 - Transient cardiomyocyte fusion regulates cardiac development in zebrafish
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Cardiac injury induces NATC reporter expression. Tg(ubb:NATC);Tg(myl7:creER) fish were 6 months old when cardiomyocyte-specific CreER was activated by intraperitoneal 4-OHT injection. 30 days later, the animals were subjected to cardiac cryoinjury or sham operation. NTR-mCherry expression (red) in sham-operated (A-C) and cryoinjured (D-H) hearts from sibling animals were visualized by immunostaining at 7 days post injury (dpi). DAPI (blue) nuclear staining shows disorganized cells in the damaged area, outlined by white dashed lines. Each image is an average intensity projection of an individual heart from a 200-300 μm thick confocal stack. A total of 4 sham and 6 cryoinjured hearts were analyzed and are shown here and in Fig. 6A. |