FIGURE

Fig. 3

ID

ZDB-FIG-180410-23

Publication

Sawamiphak et al., 2017 - Transient cardiomyocyte fusion regulates cardiac development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Membrane fusion occurs in the developing heart. Live imaging of Tg(myl7:MKATE-CAAX);Tg(myl7:H2B-GFP) embryos, in which heartbeats were blocked by morpholino-mediated tnnt2 knock-down (Supplementary Movie 1), showing establishment of a new membrane border (arrows) between cardiomyocytes 1 and 2, which initially exhibited cytoplasmic continuum. The plasma membrane border between cardiomyocytes 1 and 3 (arrowheads) dissolved and was subsequently re-established. mKATE-CAAX (red) and H2B-GFP (green) expression labeled cardiomyocyte membranes and nuclei, respectively. Timing of each still image is hour:minute. All images are 3D volume renderings of 60 μm thick confocal stacks of a representative heart showing the myocardial monolayer observed from the lumen. Examples of cytoplasmic continuum between cardiomyocytes were observed in all 6 hearts examined. Scale bar: 20 μm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.