ZFIN ID: ZDB-FIG-180404-20
Louie et al., 2017 - Temporally distinct transcriptional regulation of myocyte dedifferentiation and Myofiber growth during muscle regeneration. BMC Genomics   18:854 Full text @ BMC Genomics
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Adult

Fig. 6

Regeneration following knockdown of select differentially expressed genes. a LR regeneration assays were performed according to the schematic. b Craniectomy was performed to visualize EGFP-labeled muscle at 8 dpi. Brain was removed to allow visualization of the skull base (*) Microinjected MOs are detected throughout the entire regenerating muscle, including the distal ends (white arrow). Control MO (left) and fn1a MO (right) injected fish are pictured, Additional file 26: Figure S14 shows representative examples of injected fish with MO targeting the rest of the genes. c Quantification of LR regeneration at 8 dpi, all MOs targeting specific mRNA decreased muscle regeneration at 8 dpi compared to control MO injected fish. Values are averages ± SEM (n = 5–7) in control MO or target gene MO injected fish. *P < 0.05; **P < 0.01; *** P < 0.001; Student’s t-test. The residual muscle left following myectomy surgery (46.77 ± 4.8%, average ± SD) is represented as a grey area in C

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP zf13Tg ablation: lateral rectus Adult lateral rectus IFL
Adult regenerating tissue lateral rectus IFL
zf13Tg + MO1-fn1a ablation: lateral rectus Adult lateral rectus IFL
Adult regenerating tissue lateral rectus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf13Tg + MO1-ezh2 ablation: lateral rectus Adult lateral rectus tissue regeneration decreased efficacy, abnormal
Adult lateral rectus tissue regeneration delayed, abnormal
zf13Tg + MO1-fn1a ablation: lateral rectus Adult lateral rectus tissue regeneration decreased efficacy, abnormal
Adult lateral rectus tissue regeneration delayed, abnormal
zf13Tg + MO1-suz12a ablation: lateral rectus Adult lateral rectus tissue regeneration decreased efficacy, abnormal
Adult lateral rectus tissue regeneration delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics