ZFIN ID: ZDB-FIG-180404-19
Louie et al., 2017 - Temporally distinct transcriptional regulation of myocyte dedifferentiation and Myofiber growth during muscle regeneration. BMC Genomics   18:854 Full text @ BMC Genomics
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Adult

Fig. 5

Proliferation following knockdown of select differentially expressed genes. a LR regeneration involves a proliferative burst that generates enough cells to replace lost tissue. EdU assays were performed according to the schematic. b Gene expression (FPKM) of epigenetics selected genes, ezh2 and suz12a. c Fibronectin 1a gene expression (FPKM). d Confocal microscopy of cell proliferation in the regenerating muscle. Inset shows higher resolution detail of the box in the panel. DAPI staining (blue) shows the total number of nuclei in the muscle (left) and EdU staining (red) shows proliferating nuclei (middle). Merged panel (right). e Quantification of cell proliferation in injured LR at 48 hpi. Values are averages ± SEM (n ≥ 5) in control MO or target gene MO injected fish. Different letters (a, b, ab) in (b) and (c) indicate significant differences among time points. * P < 0.05; ** P < 0.01; Student’s t-test

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ezh2 ablation: lateral rectus Adult regenerating tissue cell population proliferation decreased occurrence, abnormal
WT + MO1-suz12a ablation: lateral rectus Adult regenerating tissue cell population proliferation decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Genomics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Genomics