Fig. 6
- ID
- ZDB-FIG-180403-23
- Publication
- Roh-Johnson et al., 2017 - Macrophage-Dependent Cytoplasmic Transfer during Melanoma Invasion In Vivo
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Macrophage Contact Precedes Cytoplasmic Transfer to Tumor Cells at the Injected Site (A) Quantification of larvae with disseminated GFP+ tumor cells that also had GFP+ tumor cells at the transplantation site 3 days prior. Number of larvae indicated in bar graph. (B) Quantification of larvae with GFP+ tumor cells at transplantation site that resulted in a disseminated GFP+, disseminated dsRed+, or no dissemination in control versus Tg(mpeg1:Cre)fh506 animals. Number of larvae indicated in bar graph. p < 0.0001, chi-square test. (C) Example of a GFP+ tumor cell at the site of transplantation at 1 dpi (left). Tumor cell is also positive in the red channel because all tumor cells were labeled with an additional red dye to better detect metastasis. Extra green fluorescence due to autofluorescence of the skin (asterisks). Right images show disseminated GFP+ tumor cell in the same larva at 4 dpi. Arrows indicate tumor cell. Inset shows zoomed region of the disseminated tumor cell. Scale bar, 30 μm. (D) Still images of a time-lapse recording of melanoma cells expressing CMV-LoxP-dsRed-STOP-LoxP-GFP (round, red cells) interacting with Tg(mfap4:tdTomato-CAAX)xt6-expressing macrophages (spindly, membrane-bound red cells, asterisks). Images are every 1 hr. Arrow marks a melanoma cell that begins to express GFP after macrophage contact. Scale bar, 30 μm. See also Movie S6. |
Reprinted from Developmental Cell, 43, Roh-Johnson, M., Shah, A.N., Stonick, J.A., Poudel, K.R., Kargl, J., Yang, G.H., di Martino, J., Hernandez, R.E., Gast, C.E., Zarour, L.R., Antoku, S., Houghton, A.M., Bravo-Cordero, J.J., Wong, M.H., Condeelis, J., Moens, C.B., Macrophage-Dependent Cytoplasmic Transfer during Melanoma Invasion In Vivo, 549-562.e6, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell