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ZFIN ID: ZDB-FIG-180330-8
Goddard et al., 2017 - Hemodynamic Forces Sculpt Developing Heart Valves through a KLF2-WNT9B Paracrine Signaling Axis. Developmental Cell   43(3):274-289.e5 Full text @ Dev. Cell
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 7

wnt9b Expression in the Developing Zebrafish Heart Is Regulated by Hemodynamic Shear Forces

(A) Whole-mount in situ hybridization for wnt9b in 48 hpf wild-type zebrafish embryos. Dotted lines outline the heart. Scale bars: top, 0.1 mm; bottom, 0.03 mm.

(B and C) In situ hybridization using RNAscope for klf2a and wnt9b in 48 hpf and 56 hpf wild-type zebrafish embryos is shown using merged z stack (B) and single slice (C) confocal analysis. Scale bar represents 100 μm and 10 μm for the magnified images in (B).

(D) In situ hybridization to detect wnt9b expression in the developing AV cushion and outflow tract of 48 hpf wild-type fish (control), silent heart (tnnt2a−/−) mutants that lack blood flow, gata1 mutants (gata1−/−) that experience low shear stress due to low blood viscosity, and klf2a mutants (klf2a−/−) is shown. Dotted lines outline the heart. Scale bars: left, 0.2 mm; right, 0.06 mm.

(E) The percentage of embryos in which wnt9b expression is normal, absent, or mis-expressed in the AV cushion or OFT is shown. The n for each group is indicated in (D). ∗∗∗∗p < 0.0001 using chi-squared analysis.

A, atrium; AV, atrioventricular; OFT, outflow tract; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
klf2a WT standard conditions Long-pec atrioventricular valve endothelial cell ISH
Long-pec bulbus arteriosus endothelial cell ISH
Long-pec endocardium cell ISH
wnt9b WT standard conditions Long-pec atrioventricular valve ISH
Long-pec atrioventricular valve endothelial cell ISH
Long-pec bulbus arteriosus ISH
Long-pec bulbus arteriosus endothelial cell ISH
Long-pec endocardium cell ISH
gata1am651/m651 standard conditions Long-pec Not Detected atrioventricular valve ISH
Long-pec Not Detected bulbus arteriosus ISH
klf2aig4/ig4 standard conditions Long-pec Not Detected atrioventricular valve ISH
Long-pec bulbus arteriosus ISH
tnnt2atc300b/tc300b standard conditions Long-pec Not Detected atrioventricular valve ISH
Long-pec Not Detected bulbus arteriosus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gata1am651/m651 standard conditions Long-pec atrioventricular valve wnt9b expression absent, abnormal
gata1am651/m651 standard conditions Long-pec bulbus arteriosus wnt9b expression absent, abnormal
klf2aig4/ig4 standard conditions Long-pec atrioventricular valve wnt9b expression absent, abnormal
Long-pec bulbus arteriosus wnt9b expression decreased amount, abnormal
tnnt2atc300b/tc300b standard conditions Long-pec atrioventricular valve wnt9b expression absent, abnormal
Long-pec bulbus arteriosus wnt9b expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 43(3), Goddard, L.M., Duchemin, A.L., Ramalingan, H., Wu, B., Chen, M., Bamezai, S., Yang, J., Li, L., Morley, M.P., Wang, T., Scherrer-Crosbie, M., Frank, D.B., Engleka, K.A., Jameson, S.C., Morrisey, E.E., Carroll, T.J., Zhou, B., Vermot, J., Kahn, M.L., Hemodynamic Forces Sculpt Developing Heart Valves through a KLF2-WNT9B Paracrine Signaling Axis, 274-289.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell