FIGURE

Fig. 4

ID

ZDB-FIG-180309-12

Publication

Sanchez et al., 2017 - Whole Genome Sequencing-Based Mapping and Candidate Identification of Mutations from Fixed Zebrafish Tissue

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Fig. 4

The fbxw7^stl64 allele phenocopies the fbxw7^vu56 allele. (A and B) stl64 mutants display more nkx2.2a+ cells in the dorsal spinal cord (SpC) relative to their WT siblings at 65 hpf. TEM analysis of the ventral (C–F) and dorsal (G–J) SpC at 8 dpf shows that fbxw7^stl64 mutants have more myelinated axons in the dorsal SpC (I) and thicker myelin in both regions (F and J). (K and L) Transient morpholino (MO) knockdown of fbxw7 in WT embryos results in mbp overexpression at 65 hpf compared to control-injected siblings. Error bars are SD. ** P < 0.01, *** P < 0.001. NS, not significant.

Expression Data

Genes:	mbpa nkx2.2a
Fish:	WT fbxw7^stl64/stl64 WT + MO1-fbxw7
Knockdown Reagent:	MO1-fbxw7
Anatomical Terms:	central nervous system spinal cord dorsal side
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fbxw7^stl64/stl64 WT + MO1-fbxw7
Knockdown Reagent:	MO1-fbxw7
Observed In:	central nervous system oligodendrocyte spinal cord central nervous system myelination spinal cord dorsal side spinal cord myelin sheath
Stage Range:	Pec-fin to Days 7-13

Phenotype Detail

Acknowledgments

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