FIGURE

Fig. 3

ID

ZDB-FIG-180302-15

Publication

Seritrakul et al., 2017 - Tet-mediated DNA hydroxymethylation regulates retinal neurogenesis by modulating cell-extrinsic signaling pathways

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Fig 3. tet2^-/-;tet3^-/- retinal cells do not undergo terminal differentiation.

(A,F) HuC/D labels RGCs and amacrine cells (ACs), which are reduced in number and located only in the central region of the INL in tet2^-/-;tet3^-/- retinae (arrow). (B,G) tet2^-/-;tet3^-/- mutant retinae almost entirely lack zpr-1⁺ red/green cones (arrow); (C,D,H,I) possess few zpr-3⁺ rods (arrow), and of those that are zpr-3⁺, outer segments are severely attenuated or almost absent (arrow). (E,J) tet2^-/-;tet3^-/- retinae also possess few zrf-1⁺ Müller glia (arrows in wild-type). In all cases, marker⁺ cells are located in the central/ventral part of the retina. (K,P) Zn8 detects neurolin, a protein enriched on RGCs and the optic nerve (arrowhead in K). (L,Q) Zn8 staining reveals the optic nerve in the choroid fissure and optic chiasm (arrow) of wild-type embryos but not in tet2^-/-;tet3^-/- mutants. (M-N) isl2b:GFP transgenics express GFP in RGCs and PRs, clearly labeling the optic nerve in whole-mount and section views (arrowhead). (R,S) The tet2^-/-;tet3^-/- optic nerve is very thin, often unilaterally formed, but, when present, correctly routed to the brain. (O,T) The isl2b:GFP signal overlaps zpr-3 (rod) marker in the cell body and outer segments in siblings (arrows). In tet2^-/-;tet3^-/-, few isl2b:GFP⁺ cells are zpr-3⁺ (arrows), further suggesting that specified cells are not terminally differentiated. Few outer segments have also formed in tet2^-/-;tet3^-/- mutants. DNA (blue), antibody stain (red). All images are 3dpf. n>5 for each marker. Dorsal is up and anterior to the left. Scale bar = 80μm in A-K, P.

Expression Data

Gene:	GFP
Antibodies:	Ab1-elavl zn-8 zpr-1 zpr-3 zrf-1
Fish:	WT zc7Tg tet2^au59/au59; tet3^au60/au60 tet2^au59/au59; tet3^au60/au60; zc7Tg
Anatomical Terms:	amacrine cell cranial nerve II green sensitive photoreceptor cell green sensitive photoreceptor cell photoreceptor outer segment Muller cell photoreceptor cell red sensitive photoreceptor cell red sensitive photoreceptor cell photoreceptor outer segment retinal ganglion cell retinal inner nuclear layer retinal rod cell retinal rod cell photoreceptor outer segment
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tet2^au59/au59; tet3^au60/au60 tet2^au59/au59; tet3^au60/au60; zc7Tg
Observed In:	cranial nerve II cranial nerve II axon eye green sensitive photoreceptor cell green sensitive photoreceptor cell photoreceptor outer segment Muller cell red sensitive photoreceptor cell red sensitive photoreceptor cell photoreceptor outer segment retina central region retina peripheral region retinal cone cell retinal ganglion cell cell morphogenesis involved in neuron differentiation retinal rod cell retinal rod cell photoreceptor outer segment
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.