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ZFIN ID: ZDB-FIG-180221-20
Piatek et al., 2017 - Ectopically expressed Slc34a2a sense-antisense transcripts cause a cerebellar phenotype in zebrafish embryos depending on RNA complementarity and Dicer. PLoS One   12:e0178219 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 75%-epiboly to Protruding-mouth

Fig. 1 Expression of Slc34a2a and related transcripts during zebrafish embryogenesis.

(A) Schematic representation of the Slc34a2a, Slc34a2a(as) and Rbpja loci. The antisense transcript Slc34a2a(as) is depicted in red. (B) RT-qPCR analysis of Slc34a2a, Slc34a2a(as) and Rbpja transcripts including the paralog Slc34a2b. Based on negative controls using RNA as an input, the detection limit was set at a δCt of 12 which is in agreement with ISH results. (C) Demonstration of Slc34a2a, Slc34a2a(as), Rbpja, Slc34a2b and Shh (Sonic Hedgehog) transcripts at progressing stages of development by whole mount ISH.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
rbpja AB standard conditions 75%-epiboly whole organism ISH
Prim-5 whole organism ISH
Long-pec midbrain ISH
Long-pec otic vesicle ISH
Protruding-mouth midbrain ISH
Protruding-mouth otic vesicle ISH
slc34a2a AB standard conditions 75%-epiboly Not Detected whole organism ISH
Prim-5 Not Detected whole organism ISH
Long-pec endoderm ISH
Protruding-mouth endoderm ISH
slc34a2aas AB standard conditions 75%-epiboly whole organism ISH
Prim-5 endoderm ISH
Prim-5 head ISH
Prim-5 pharynx ISH
Prim-5 primordial hindbrain channel ISH
Prim-5 primordial midbrain channel ISH
Long-pec endoderm ISH
Long-pec head ISH
Long-pec pharynx ISH
Long-pec primordial hindbrain channel ISH
Long-pec primordial midbrain channel ISH
Protruding-mouth endoderm ISH
Protruding-mouth head ISH
Protruding-mouth pharynx ISH
Protruding-mouth primordial hindbrain channel ISH
Protruding-mouth primordial midbrain channel ISH
slc34a2b AB standard conditions 75%-epiboly whole organism ISH
Prim-5 whole organism ISH
Long-pec endoderm ISH
Long-pec otic vesicle ISH
Long-pec pharynx ISH
Protruding-mouth endoderm ISH
Protruding-mouth otic vesicle ISH
Protruding-mouth pharynx ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One