Fig. 5

Morpholino knockdown of Slc34a2a(as) and Dicer.

A) RT-qPCR quantification of Slc34a2a, Slc34a2a(as) and Slc34a2b after splice site morpholino injection at 24 hpf. Wild type non injected controls, light blue bars; 5 ng splice-site MO injected embryos are in dark blue; 5 ng scrambled MO injected embryos are in blue. B) Phenotypic characterization of MO injected embryos at 24 and 48 hpf. The injected oligonucleotides and quantities are indicated below the bars. Phenotypic scaling was performed as described in Fig 2<. C) Rescue of cerebellum development by Dicer knockdown. Phenotypic assessment of embryos injected with combinations of Dicer MO, p53 MO and Slc34a2a. D) 48 hpf zebrafish embryos injected with Dicer MO, p53 MO and Slc34a2a as indicated in the pictures. In the upper panel, heads with red arrows indicating the cerebellum are shown; the lower panel shows ISH of embryos with an Eng2 probe.