The HSPC defects in rad51sa23805 fish are rescued in a p53 mutant background. (A) Representative images of 4-dpf embryos resulting from in-crosses of p53+/− rad51+/− parents stained using a cmyb-specific probe. The total number of embryos used (all genotypes) n = 237 from four clutches. For information about all genotypes, see SI Appendix, Table S3. Arrows indicate HSPCs. (Magnification, 100×.) (B) Percentage of BrdU+ cells in the kidney at 4 mpf at 1 dpi. Two-sided Student’s t test, np53+/+ rad51+/+ = 5, np53−/− rad51−/− = 5. (C) Number of total cells per kidney at 4 mpf quantified using a hemocytometer. Analysis using one-way ANOVA [F(3, 43) = 10.45, P < 0.0001], individual P values shown in the figure are from Tukey’s post hoc test, np53+/+ rad51+/+ = 16, np53+/+ rad51−/− = 16, np53−/− rad51+/+ = 6, np53−/− rad51−/− = 8. Bars represent mean ± SEM in B and C; n.s., not significant.