ZFIN ID: ZDB-FIG-180209-4
Botthof et al., 2017 - Loss of the homologous recombination gene rad51 leads to Fanconi anemia-like symptoms in zebrafish.. Proceedings of the National Academy of Sciences of the United States of America   114(22):E4452-E4461 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Adult

Fig. 5

The HSPC defects in rad51sa23805 fish are rescued in a p53 mutant background. (A) Representative images of 4-dpf embryos resulting from in-crosses of p53+/− rad51+/− parents stained using a cmyb-specific probe. The total number of embryos used (all genotypes) n = 237 from four clutches. For information about all genotypes, see SI Appendix, Table S3. Arrows indicate HSPCs. (Magnification, 100×.) (B) Percentage of BrdU+ cells in the kidney at 4 mpf at 1 dpi. Two-sided Student’s t test, np53+/+ rad51+/+ = 5, np53−/− rad51−/− = 5. (C) Number of total cells per kidney at 4 mpf quantified using a hemocytometer. Analysis using one-way ANOVA [F(3, 43) = 10.45, P < 0.0001], individual P values shown in the figure are from Tukey’s post hoc test, np53+/+ rad51+/+ = 16, np53+/+ rad51−/− = 16, np53−/− rad51+/+ = 6, np53−/− rad51−/− = 8. Bars represent mean ± SEM in B and C; n.s., not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myb rad51sa23805/sa23805 ; tp53zdf1/zdf1 standard conditions Day 4 hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rad51sa23805/sa23805 ; tp53zdf1/zdf1 standard conditions Day 4 blood island hematopoietic stem cell amount, ameliorated
Adult head kidney hematopoietic stem cell amount, ameliorated
Adult head kidney hematopoietic stem cell proliferation rate, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA