ZFIN ID: ZDB-FIG-180209-3
Botthof et al., 2017 - Loss of the homologous recombination gene rad51 leads to Fanconi anemia-like symptoms in zebrafish.. Proceedings of the National Academy of Sciences of the United States of America   114(22):E4452-E4461 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 4

The rad51sa23805 HSPC defect starts during embryonic development. (A) ISH using a cmyb-specific probe at 2 dpf; the arrow shows HSPCs. Representative images of the three different staining categories are shown (i) and a quantification of the different genotypes (ii) n = 119 from two clutches. (B) ISH using a cmyb-specific probe at 4 dpf; the arrow shows HSPCs. Representative images of the three different staining categories are shown (i) and a quantification of the different genotypes (ii), n = 120 from two clutches. (C) Quantification of BrdU+ cells in the tail at 2 dpf. Two-sided Student’s t test, P = 0.042, n+/+ = 3, n−/− = 3. (D) Quantification of BrdU+ cells in the CHT at 4 dpf. Two-sided Student’s t test, n+/+ = 4, n−/− = 4. Bars represent mean ± SEM in C and D. n.s., not significant. (E) Representative images of TUNEL-stained 2 dpf embryos from a rad51+/− in-cross. Dotted lines indicate the area of the CHT that was scored. Arrows indicate TUNEL+ cells. (F) Quantification of three clutches of TUNEL-stained 2 dpf rad51+/− in-crosses. Each clutch was scored blindly and consisted of 10+/+ and 10−/− embryos each. Shown is the mean of all clutches ± SEM. (Magnification, 100× in all images.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myb rad51sa23805/+ standard conditions Long-pec hematopoietic stem cell ISH
Day 4 hematopoietic stem cell ISH
rad51sa23805/sa23805 standard conditions Long-pec hematopoietic stem cell ISH
Day 4 hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rad51sa23805/sa23805 standard conditions Long-pec blood island hematopoietic stem cell proliferation decreased rate, abnormal
Long-pec blood island hepatoblast apoptotic process increased occurrence, abnormal
Long-pec hematopoietic stem cell decreased amount, abnormal
Day 4 blood island hematopoietic stem cell proliferation decreased rate, abnormal
Day 4 hematopoietic stem cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA