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ZFIN ID: ZDB-FIG-180208-2
Louw et al., 2018 - Compound heterozygous loss-of-function mutations in KIF20A are associated with a novel lethal congenital cardiomyopathy in two siblings. PLoS Genetics   14:e1007138 Full text @ PLoS Genet.
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 6

Histology and cardiac function evaluation of zebrafish kif20a morphants.

H&E staining of zebrafish embryos injected with control MO and kif20a MO at 4 dpf. Upper panel: Bright-field images of control and kif20a morphants at 4 dpf showing the section locations 1 and 2. Middle panel: Representative H&E staining images of control and kif20a morphants at position 1 and 2 in 10x and 20x magnification. White arrows in position 1 indicate the aortic arch, and white arrows in position 2 indicate gut. Lower panel: Quantification of ventricle and atrium thickness using H&E staining data above. Data are represented as mean ± SD. Stars represent the results of one-way ANOVA-Dunnett’s post hoc test (*p<0.05, **p < 0.01, ns, not significant). (B) Cardiac function analysis. Left panel: Heart rate in beats per minute is compared in control and kif20a morphants at 3 dpf and 4 dpf. A significant increase in heart rate is observed in the morphants at 4 dpf, most likely due to progressive cardiac failure. Right panel: Fractional shortening measured at 4dpf is compared in control and kif20a morphants respectively in the atrium and ventricle. Although a significant increased fractional shortening is present in the morphants, more outliers are seen, suggesting systolic failure.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-kif20a standard conditions Protruding-mouth heart contraction rate, normal
Day 4 atrioventricular valve morphology, normal
Day 4 atrium increased thickness, abnormal
Day 4 blood accumulation atrium, abnormal
Day 4 bulbus arteriosus decreased size, abnormal
Day 4 cardiac ventricle increased thickness, abnormal
Day 4 heart contraction increased rate, abnormal
Day 4 heart decreased functionality, abnormal
Day 4 heart decreased size, abnormal
Day 4 pericardium edematous, abnormal
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.