FIGURE

Fig. 1

ID

ZDB-FIG-180112-4

Publication

Yokokura et al., 2017 - The Short-Stature Homeobox-Containing Gene (shox/SHOX) Is Required for the Regulation of Cell Proliferation and Bone Differentiation in Zebrafish Embryo and Human Mesenchymal Stem Cells.

Other Figures

Fig. 1

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Fig. 1

Figure 1. Translational blockade of zebrafish shox reduced cell proliferation and specific gene expression of skeletal progenitors in developing embryos. (A) Analyses of cell proliferation and cell death of shox morpholino oligo (MO) or control MO (ctr MO)-injected 22 hpf embryos. Bars, 100 µm. Representative results of phospho-Histone H3-staining and active-caspase-3-staining. Quantification data of phospho-Histone H3-staining of ctr MO-injected embryos (n = 5) and shox MO-injected embryos (n = 9). *P < 0.05. Quantification data of active caspase-3-staining of ctr MO-injected embryos (n = 3) and shox MO-injected embryos (n = 9). (B) Whole-mount in situ hybridization analysis and whole-mount immunostaining of 24 hpf embryos. The cRNA probe either for dlx2 mRNA, pax2a, or ntl mRNA was used. The arrows indicate domains with dlx2 positive signals. Whole-mount immunostaining analysis of myosin heavy chain (MyHC) (green) and Hoechst33342 (blue) was conducted. Representative results are displayed and the penetrance is shown in the lower right corner of each panel.

Expression Data

Genes:	dlx2a pax2a tbxta
Fish:	WT WT + MO1-shox
Knockdown Reagent:	MO1-shox
Anatomical Terms:	neuron notochord posterior region pharyngeal mesoderm
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-shox
Knockdown Reagent:	MO1-shox
Observed In:	apoptotic process cell population proliferation pharyngeal mesoderm skeletal muscle fiber development
Stage Range:	26+ somites to Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)