FIGURE

Fig. S1

ID

ZDB-FIG-180112-13

Publication

Facchinello et al., 2017 - nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor

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Fig. S1

(A): qRT-PCR of gr mRNA in 5-dpf mutant larvae compared to control shows a statistically significant reduction of gr expression. Values represent the mean ± SEM. Asterisks indicate that expression levels are significantly different from the control: ***P < 0.001. Data were generated from four biological replicates. (B): Representative images of 5-dpf control gr^+/+ and mutant gr^-/- larvae after exposure to VBA stimulus. gr^-/- mutants appear darker in comparison to control. (C): Representative gel image of PCR genotyping using genomic DNA from tail fins of adults born from a cross between gr heterozygotes. (D): Western blot of liver proteins from 8-month-old gr^-/- and gr^+/+ zebrafish showing disappearance of the protein band with respect to control.

Expression Data

Gene:	nr3c1
Fish:	WT nr3c1^ia30/ia30 nr3c1^s357/s357
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nr3c1^ia30/ia30
Observed In:	whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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