IMAGE

Fig. S1

ID

ZDB-IMAGE-180112-17

Genes

nr3c1

Publication

Facchinello et al., 2017 - nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor

All Figures

Figures for Facchinello et al., 2017

Image

Figure Caption

Fig. S1

(A): qRT-PCR of gr mRNA in 5-dpf mutant larvae compared to control shows a statistically significant reduction of gr expression. Values represent the mean ± SEM. Asterisks indicate that expression levels are significantly different from the control: ***P < 0.001. Data were generated from four biological replicates. (B): Representative images of 5-dpf control gr^+/+ and mutant gr^-/- larvae after exposure to VBA stimulus. gr^-/- mutants appear darker in comparison to control. (C): Representative gel image of PCR genotyping using genomic DNA from tail fins of adults born from a cross between gr heterozygotes. (D): Western blot of liver proteins from 8-month-old gr^-/- and gr^+/+ zebrafish showing disappearance of the protein band with respect to control.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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