ZFIN ID: ZDB-FIG-180109-12
Turrini et al., 2017 - Optical mapping of neuronal activity during seizures in zebrafish. Scientific Reports   7:3025 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 4

Fig. 1

Brain activity recording with GCaMP6s. (a) Fluorescence image of the encephalon of a 4 dpf larva expressing GCaMP6s under elavl3 promoter. Scale bar: 100 µm. The four main zebrafish brain regions used for further analysis in the paper are highlighted (Telencephalon, blue; Optic tectum, orange; Cerebellum, yellow; Medulla, purple). (b) Time trace of fluorescence (ΔF/F0, see Methods for detail) in control conditions and after addition of 15 mM PTZ (at the time indicated). The fluorescence data are integrated over the whole brain of the larva and sampled at 5 Hz. The inset shows a shorter time interval of the trace to better display calcium peak shape and regularity. (c) ΔF/F0 trace of an experiment in which tricaine (160 mg/L) was added at the time indicated and the subsequent decay of activity was monitored. The ΔF/F0 values become negative after tricaine addition since the F0 reference level was measured in control conditions (see Methods for details). (d) ΔF/F0 trace showing sharp brain activity decay in a larva simultaneously treated with 15 mM PTZ and 5 μM TTX. After approximately 35 min upon drugs application, TTX action totally suppressed neuronal seizure activity induced by PTZ.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GCaMP fi1Tg control Day 4 cerebellum neuron IFL
Day 4 medulla oblongata neuron IFL
Day 4 optic tectum neuron IFL
Day 4 telencephalon neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fi1Tg chemical treatment by environment: pentetrazol Day 4 brain calcium-mediated signaling increased process quality, abnormal
Day 4 brain transmission of nerve impulse increased process quality, abnormal
fi1Tg chemical treatment by environment: tetrodotoxin, chemical treatment by environment: pentetrazol Day 4 brain calcium-mediated signaling decreased process quality, abnormal
Day 4 brain transmission of nerve impulse decreased process quality, abnormal
fi1Tg chemical treatment by environment: tricaine methanesulfonate Day 4 brain calcium-mediated signaling decreased process quality, abnormal
Day 4 brain transmission of nerve impulse decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.