FIGURE

Fig. S2

ID
ZDB-FIG-180108-25
Publication
Patra et al., 2017 - The zebrafish ventricle: A hub of cardiac endothelial cells for in vitro cell behavior studies
Other Figures
All Figure Page
Back to All Figure Page
Fig. S2

Endothelial cell density in 8 months old mouse and zebrafish ventricles. (a) Representative confocal images of sagittal cryosections through 8 months old mouse hearts stained for ?-actinin (green; marking cardiomyocytes), CD31 (red; marking the cell membrane of endothelial cells) and DAPI (blue; staining the nuclei). (b) Representative confocal images of sagittal cryosections through 8 mpf Tg(kdrl:Hsa.HRAS-mCherry) zebrafish hearts stained for ?-actinin (green; marking cardiomyocytes), mCherry (red; marking endothelial cell membrane) and DAPI (blue; staining the nuclei). White arrowheads point to bigger coronary vessels in the outer compact layer; white arrows point to dense capillaries in the inner compact layer. (c) Quantification of the area covered by endothelial cells (as percentage of total cardiac tissue area). 10 sections from each heart from three zebrafish and three mice were analyzed (meanąSEM). One way ANOVA followed by Bonferroni?s post-hoc test (GraphPad Prism) was performed to evaluate statistical significance of differences. p < 0.05 was considered statistically significant. *** corresponds to P<0.001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.