Fig. 5
- ID
- ZDB-FIG-171228-49
- Publication
- Rességuier et al., 2017 - Specific and Efficient Uptake of Surfactant-Free Poly(Lactic Acid) Nanovaccine Vehicles by Mucosal Dendritic Cells in Adult Zebrafish after Bath Immersion
- Other Figures
- All Figure Page
- Back to All Figure Page
Active nanoparticles (NPs) internalization in cells of mucosae, kidneys, and liver. (A) NP internalization score, assessed by imaging in flow, in dissected organs from adult zebrafish exposed to 0.05% red fluorescent NPs (red histograms). As a negative control for internalization (blue histograms), PLA-NPs were added at 4°C just before acquisition on the cell suspension from fish unexposed to NP. Whereas most NPs are found at or outside the cell membrane in the negative controls (internalization scores <−1), a high majority of NPs is detected inside the cells of NP-treated fish (internalization scores >2) in all analyzed organs. (B–D) Representative images of NPs in kidney (B,C) or gill (D) cell suspensions, from NP-exposed fish (B,D) or control conditions (C), which display membrane processes reminiscent of dendrites. Total number of analyzed cells (NP 0.05%/4°C control): gills (three independent acquisitions) 1312/4355, skin (two independent acquisitions) 345/4185, liver (two independent acquisitions) 1431/2651, and kidney (one acquisition) 117/3180. One fish per acquisition. Error bars: SD. |