FIGURE

Fig. 3

ID

ZDB-FIG-171228-48

Publication

Rességuier et al., 2017 - Specific and Efficient Uptake of Surfactant-Free Poly(Lactic Acid) Nanovaccine Vehicles by Mucosal Dendritic Cells in Adult Zebrafish after Bath Immersion

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Fig. 3

Nanoparticles (NPs) reach the circulatory system and the liver. (A,B′) Representative confocal images of gills from transgenic fli:GFP adults bathed for 24 h in 0.05% red fluorescent NPs. Images were acquired in 40-μm-thick whole-body cryosections, stained with DAPI [blue in (A)]. NPs are found in the lumen of gill arch (A) and gill filament (B) blood vessels, which are delimited by green endothelial cells. NPs were also detected inside endothelial cells [arrow in (B)]. (B′) These observations are confirmed by the orthogonal view realized in the area between the two dotted lines in (B). (C) Representative confocal image of the liver of wild-type adults exposed to 0.01% red fluorescent NPs. Images were acquired in 40-μm-thick whole-body cryosections, stained with phalloidin (green) and DAPI (blue). NPs are highly concentrated in cells close to sinusoids (s) and displaying an oblong nucleus (arrows). pL, primary lamellae; sL, secondary lamellae; Bc, branchial cavity; B, blood vessel; Ga, gill arch; C, cartilage; Hp, hepatocytes. Scale bar: 10 μm (B,B′), 20 μm (A,C).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol