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ZFIN ID: ZDB-FIG-171206-11
Berres et al., 2017 - Transcriptome Profiling Identifies Ribosome Biogenesis as a Target of Alcohol Teratogenicity and Vulnerability during Early Embryogenesis. PLoS One   12:e0169351 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 1

zrps3a, zrpl5a or zrpl11 knockdown heightens vulnerability to alcohol-induced craniofacial deficits in zebrafish embryos.

(A-E) Untreated embryos (A) had normal development of cranial cartilage elements, and these were largely unaffected by treatment with a nonsense morpholino (B) or morpholinos directed against zrps3a (C), zrpl5a (D), or zrpl11 (E). (F-J) Alcohol treatment modestly reduced the size and shape of cranial cartilage elements in otherwise normal embryos. Treatment with a nonsense morpholino (G) did not further worsen cranial development. However, the combination of alcohol with morpholino directed against zrps3a (H), zrpl5a (I), or zrpl11 (J) resulted in ablation of many cranial cartilage elements and reduced ocular size. Embryos are either 4 dpf (A-D, F-I) or 3 dpf (E, J). All views are ventral with equivalent magnification. Abbreviations used: c, cardiac edema; cb, ceratobranchial; ch, ceratohyal; ep, ethmoid plate; m, Meckel’s cartilage.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: ethanol Day 4 embryonic cranial skeleton morphogenesis decreased process quality, abnormal
Day 4 embryonic cranial skeleton morphogenesis process quality, normal
Day 4 embryonic neurocranium morphogenesis process quality, normal
Day 4 embryonic viscerocranium morphogenesis process quality, normal
Day 4 ethmoid cartilage decreased size, abnormal
Day 4 ethmoid cartilage malformed, abnormal
Day 4 eye decreased size, abnormal
Day 4 eye increased distance eye, abnormal
Day 4 mandibular arch skeleton malformed, abnormal
WT + MO1-rpl11 control Protruding-mouth embryonic cranial skeleton morphogenesis process quality, normal
Protruding-mouth embryonic neurocranium morphogenesis process quality, normal
Protruding-mouth embryonic viscerocranium morphogenesis process quality, normal
WT + MO1-rpl11 chemical treatment by environment: ethanol Protruding-mouth embryonic cranial skeleton morphogenesis decreased process quality, exacerbated
Protruding-mouth embryonic neurocranium morphogenesis decreased process quality, exacerbated
Protruding-mouth heart edematous, exacerbated
Protruding-mouth mandibular arch skeleton malformed, exacerbated
Protruding-mouth Meckel's cartilage decreased length, exacerbated
Protruding-mouth Meckel's cartilage malformed, exacerbated
Protruding-mouth neurocranium malformed, exacerbated
WT + MO1-rpl5a control Day 4 embryonic cranial skeleton morphogenesis process quality, normal
Day 4 embryonic neurocranium morphogenesis process quality, normal
Day 4 embryonic viscerocranium morphogenesis process quality, normal
WT + MO1-rpl5a chemical treatment by environment: ethanol Day 4 embryonic cranial skeleton morphogenesis decreased process quality, exacerbated
Day 4 embryonic neurocranium morphogenesis decreased process quality, exacerbated
Day 4 mandibular arch skeleton malformed, exacerbated
Day 4 neurocranium malformed, exacerbated
WT + MO1-rps3a control Day 4 embryonic cranial skeleton morphogenesis process quality, normal
Day 4 embryonic neurocranium morphogenesis process quality, normal
Day 4 embryonic viscerocranium morphogenesis process quality, normal
WT + MO1-rps3a chemical treatment by environment: ethanol Day 4 embryonic cranial skeleton morphogenesis decreased process quality, exacerbated
Day 4 embryonic neurocranium morphogenesis decreased process quality, exacerbated
Day 4 mandibular arch skeleton malformed, exacerbated
Day 4 neurocranium malformed, exacerbated
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One