ZFIN ID: ZDB-FIG-171129-15
Sharma et al., 2016 - ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions. Scientific Reports   6:37172 Full text @ Sci. Rep.
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec

Fig. 2

Expression of ELMO1 is observed within the zebrafish pronephros but remains unaltered under hyperglycaemic conditions as compared to normoglycaemic fish.

(A) Illustrates the position of the pronephros in a Tg(wt1b:EGFP) embryo (48 hpf), visible from the dorsal view. The renal structure is composed of two tubules that are fused to form a single glomerulus (white arrow) via a neck (white hashtag). Both the scale bars represent 200 μm. (B) No change in relative ELMO1 expression was observed within the zebrafish pronephros under hyperglycaemic conditions (4 ng morpholino injection against insulin promotor factor 1, PDX1). Co. Mo. indicates 4 ng control morpholino. Pronephric cells were isolated for mRNA extraction via FACS sorting from Tg(wt1b:EGFP) embryos for cDNA synthesis and subsequent real-time qPCR analysis. (C) tabulates the mean Cp (crossing point of the amplifying fluorescence exceeding the background fluorescence) values of ELMO1 (target) and β-actin 1 (reference) expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP li1Tg standard conditions Long-pec pronephric glomerulus IFL
Long-pec pronephric podocyte IFL
Long-pec pronephric tubule IFL
elmo1 li1Tg control Long-pec pronephros RTPCR
li1Tg + MO1-pdx1 standard conditions Long-pec pronephros RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
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