FIGURE

Fig. 6

ID

ZDB-FIG-171127-29

Publication

Ota et al., 2016 - Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish

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Fig. 6

Expression of neural genes in the midbrain and the hindbrain of Tg[epdr1-hs:eGFP] embryos.

The expression of neural marker genes was examined by whole-mount in situ hybridization using the indicated anti-sense RNA probes at 30 hpf. The expression of pax2a in the MHB and the optic stalk of the homozygous Tg[epdr1-hs:eGFP] embryo was slightly decreased compared with that of the wild-type or the heterozygous Tg[epdr1-hs:eGFP] embryos. The expression of atp1a3a and zic3 was not affected.

Expression Data

Genes:	atp1a3a pax2a zic3
Fish:	WT epdr1^{uy220Tg/uy220Tg} epdr1^uy220Tg/+
Anatomical Terms:	midbrain hindbrain boundary nervous system optic stalk
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	epdr1^{uy220Tg/uy220Tg}
Observed In:	midbrain hindbrain boundary optic stalk
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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