FIGURE

Fig. 3

ID

ZDB-FIG-171127-26

Publication

Ota et al., 2016 - Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

eGFP expression visualized by the endogenous pax2a enhancer activity.

(a,c) The MHB (asterisk) of a heterozygous Tg[pax2a-hs:eGFP] embryo at 30 hpf. (b,d) The midbrain and hindbrain of a homozygous Tg[pax2a-hs:eGFP] embryo at 30 hpf. (e,f) The spinal cord neurons (sc) and pronephric duct (pd) of heterozygous (e) and homozygous (f) Tg[pax2a-hs:eGFP] embryos at 30 hpf. (g) The MHB of the heterozygous Tg[pax2a-hs:eGFP] embryo at 72 hpf. (h) The midbrain and hindbrain of the homozygous Tg[pax2a-hs:eGFP] embryo at 72 hpf. (i,j) The optic nerves of heterozygous (i) and homozygous (j) Tg[pax2a-hs:eGFP] embryos at 72 hpf. (k,l) The thyroids of heterozygous (k) and homozygous (l) Tg[pax2a-hs:eGFP] embryos at 72 hpf. All data were obtained by confocal microscopy. (a,b,e,f) Genotyping was performed using genomic PCR. Lateral view with anterior to the left and dorsal to the top. (c,d,g,h) Dorsal view with anterior to the left. (i–l) Ventral view with anterior to the left.

Expression Data

Gene:	EGFP
Fish:	pax2a^{uy221Tg/uy221Tg} pax2a^uy221Tg/+
Anatomical Terms:	cranial nerve II midbrain midbrain hindbrain boundary optic chiasm pronephric duct spinal cord interneuron thyroid follicle
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax2a^{uy221Tg/uy221Tg}
Observed In:	cranial nerve II neuron projection midbrain midbrain hindbrain boundary optic chiasm optic chiasm cranial nerve II thyroid follicle
Stage Range:	Prim-15 to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.