FIGURE

Fig. 4

ID
ZDB-FIG-171113-49
Publication
Kotb et al., 2016 - Knockdown of ApoL1 in Zebrafish Larvae Affects the Glomerular Filtration Barrier and the Expression of Nephrin
Other Figures
(all 10)
All Figure Page
Back to All Figure Page
Fig. 4

zApoL1 KD changes the morphology of the glomerulus.

zApoL1 was knocked down in larvae expressing EGFP in podocytes by zApoL1 TBM (A-D), zApoL1 SBM exon2 (E-H) and zApoL1 SBM exon6 (I-L) morpholinos. Paraffin sections of the larvae were stained with Alexa 546-phalloidin for F-actin and DAPI for the nuclei. The KD with three different morpholinos against zApoL1 resulted in a similar phenotype, comprising dilation of Bowman’s space (arrows in B, F, J), collapse and malformation of the glomerular tuft (asterisks in B, F, J). CtrlMO and Control larvae are shown in panel M-T. Scale bars represent 20 μm.
Expression Data
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Day 4

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.