FIGURE

Fig. 5

ID

ZDB-FIG-171101-7

Publication

Lauterbach et al., 2015 - Fast Calcium Imaging with Optical Sectioning via HiLo Microscopy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Fast calcium imaging at 100 fps with HiLo microscopy reveals different dynamics in soma and initial segment.

(A) HiLo image of motor neurons expressing GCaMP5G with ROIs. Scale bar 5 μm. (B) Time course of calcium signals in soma, initial segment and axon, recorded at 100 fps. In the axon (ROI 1, gray) and the initial segments (ROI 4, light blue; ROI 5, magenta) a much faster rise is observed than in the somata (ROI 2, orange; ROI 3, dark blue). Somatic signals show a linear rise and a mono-exponential decay (Inset: blue somatic signal with red dotted fit, decay time 1.7 s, R² = 0.998). (C) The kinetics of calcium transients in different compartments were stable over time as shown with a recording performed 2.5 min later.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One