FIGURE

Fig. 3

ID

ZDB-FIG-171101-6

Publication

Lauterbach et al., 2015 - Fast Calcium Imaging with Optical Sectioning via HiLo Microscopy

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Fig. 3

HiLo microscopy enhances calcium signals.

(A) In vivo HiLo image of motor neurons of a zebrafish embryo expressing GCaMP5G in soma (ROI1), axons (ROIs 2–4), or background (ROIs 5–7). Scale bar 5 μm. (B) ΔF/F time series in the soma (ROI 1) shows that HiLo mode leads to a larger signal than UWF mode. (C) For axons (ROI 4) the gain in HiLo mode is even larger. Acquisition rate was 25fps.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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