FIGURE

Fig. 4

ID

ZDB-FIG-171030-30

Publication

Lou et al., 2015 - Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest

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Fig. 4

No apparent alterations in cell proliferation and cell death in arch-forming regions of mutant embryos. a to l Cell proliferation at 36 hpf was analyzed through BrdU incorporation. Neural crest cells were marked by sox10:EGFP transgene and revealed by immunostaining with anti-GFP antibody. Proliferating cells were marked by BrdU then revealed by immunostaining with anti-BrdU antibody. Lateral views with anterior to left. m to t Cell death at 36 hpf was analyzed through TUNEL assay. Lateral views with anterior to left. u Quantification of BrdU-positive cell in 2nd arch (dotted squares in a, d, g and j) in control and mutant embryos. Bars represent the SD. v Quantification of TUNEL positive cell in one side of pharyngeal arches in control and mutant embryos at 36 hpf. Bars represent the SD. Scale bars, 100 um

Expression Data

Gene:	EGFP
Fish:	ir937Tg med14^s231/s231; ir937Tg smarca4a^s481/s481; ir937Tg med14^s231/s231; smarca4a^s481/s481; ir937Tg
Anatomical Term:	neural crest
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.