PUBLICATION

Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest

Authors

Lou, X., Burrows, J.T., Scott, I.C.

ID

ZDB-PUB-151111-3

Date

2015

Source

BMC Developmental Biology 15: 41 (Journal)

Registered Authors

Burrows, Jeff, Lou, Xin, Scott, Ian

Keywords

Neural crest, Mediator complex, BAF complex, Brg1, Craniofacial defect, Med14, Jaw development

MeSH Terms

Zebrafish/embryology*
Jaw Abnormalities/genetics
Maxillofacial Development/genetics*
Maxillofacial Development/physiology
Cell Proliferation
Mediator Complex/genetics*
Mediator Complex/metabolism
Cell Differentiation/genetics
Adaptor Proteins, Signal Transducing/genetics*
Adaptor Proteins, Signal Transducing/metabolism
Cells, Cultured
Cell Movement
Neural Crest/cytology*
Embryo, Nonmammalian/embryology
Jaw/embryology
Osteogenesis/genetics*
Osteogenesis/physiology
Animals
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Gene Regulatory Networks/genetics
Gene Expression Regulation, Developmental

(all 22)

PubMed

26553192 Full text @ BMC Dev. Biol.

Abstract

Background An intricate gene regulatory network drives neural crest migration and differentiation. How epigenetic regulators contribute to this process is just starting to be understood.

Results We found that mutation of med14 or brg1 in zebrafish embryos resulted in a cluster of neural crest cell-related defects. In med14 or brg1 mutants, neural crest cells that form the jaw skeleton were specified normally and migrated to target sites. However, defects in their subsequent terminal differentiation were evident. Transplantation experiments demonstrated that med14 and brg1 are required directly in neural crest cells. Analysis of med14; brg1 double mutant embryos suggested the existence of a strong genetic interaction between members of the Mediator and BAF complexes.

Conclusions These results suggest a critical role for Mediator and BAF complex function in neural crest development, and may also clarify the nature of defects in some craniofacial abnormalities.

Genes / Markers

Figures

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Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
med14^s231/s231	standard conditions	14-19 somites	migratory neural crest	ISH	Fig. 3
med14^s231/s231; smarca4a^s481/s481	standard conditions	14-19 somites	migratory neural crest	ISH	Fig. 3
smarca4a^s481/s481	standard conditions	14-19 somites	migratory neural crest	ISH	Fig. 3
WT	control	14-19 somites	migratory neural crest	ISH	Fig. 3
med14^s231/s231	standard conditions	Prim-5	pharyngeal arch	ISH	Fig. 3
med14^s231/s231; smarca4a^s481/s481	standard conditions	Prim-5	pharyngeal arch	ISH	Fig. 3
smarca4a^s481/s481	standard conditions	Prim-5	pharyngeal arch	ISH	Fig. 3
WT	control	Prim-5	pharyngeal arch	ISH	Fig. 3
ir937Tg	control	14-19 somites	migratory neural crest	IFL	Fig. 3
ir937Tg	control	Prim-5	neural crest	IFL	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	basihyal cartilage absent, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	ceratobranchial 1 cartilage absent, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	ceratobranchial 2 cartilage absent, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	ceratobranchial 3 cartilage absent, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	ceratobranchial 4 cartilage absent, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	ceratobranchial 5 cartilage absent, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	ceratohyal cartilage decreased size, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	ethmoid cartilage morphology, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	hyosymplectic cartilage absent, abnormal	Fig. 1
med14^s231/+; smarca4a^s481/s481	standard conditions	Day 4	Meckel's cartilage decreased size, abnormal	Fig. 1

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ir937Tg	Tg(-7.2sox10:EGFP)	Transgenic Insertion
s231		Point Mutation	med14
s481		Point Mutation	smarca4a
twu34Tg	Tg(myl7:EGFP)	Transgenic Insertion

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
ir937Tg
med14^s231/s231
med14^s231/s231; ir937Tg
med14^s231/s231; smarca4a^s481/s481
med14^s231/s231; smarca4a^s481/s481; ir937Tg
med14^s231/s231; smarca4a^s481/+
med14^s231/+; smarca4a^s481/s481
smarca4a^s481/s481
smarca4a^s481/s481; ir937Tg
WT

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Lou et al., 2015 ZDB-PUB-151111-3 PMID:26553192

Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest

Lou et al., 2015

ZDB-PUB-151111-3
PMID:26553192