Fig. 3
- ID
- ZDB-FIG-171024-16
- Publication
- Cambier et al., 2017 - Phenolic Glycolipid Facilitates Mycobacterial Escape from Microbicidal Tissue-Resident Macrophages
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Mm PGL Recruits Monocytes through STING-Dependent ccl2 Induction (A) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type or Sting-deficient fish with 250–300 wild-type Mm. Student’s unpaired t test. (B and C) In situ hybridizations against zebrafish ccl2 mRNA following hindbrain ventricle infections with 80 wild-type Mm into wild-type (B) or Sting-deficient (C) zebrafish. Black arrows, ccl2 mRNA-positive phagocytes; white arrows ccl2 mRNA-negative phagocytes. Scale bar, 50μm. Results representative of three independent experiments. (D) Mean resident macrophage and monocyte recruitment from 5 to 180 mpi in the HBV of wild-type or Sting-deficient fish after infection with 80 wild-type Mm. (E) Mean resident macrophage and monocyte recruitment from 5 to 180 mpi in the HBV of wild-type or Sting-deficient fish after infection with 80 Mm - PDIM-. (F) Percentage of infected (black) or uninfected (gray) wild-type or Sting-deficient fish 5 dpi with 1-3 wild-type Mm into the HBV. n = number of larvae per group. Results representative of two independent experiments. Significance testing done using Fisher’s exact test. (G) ccl2, ifnΦ1, ifnΦ2, and ifnΦ3 mRNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type fish with 250–300 wild-type Mm. Significance testing done using Student’s unpaired t test for each gene. p = 0.002 for ccl2, all other comparisons not significant. (H) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of wild-type fish after infection with 80 wild-type or ESX-1-deficient (ESX1−) Mm. (I) Percentage of infected (black) or uninfected (gray) wild-type fish 5 dpi of 1–3 wild-type, ESX1−, or PGL− Mm into the HBV. n = number of larvae per group. Significance testing done using Fisher’s exact test for comparisons shown. ∗∗p < 0.01, ∗∗∗p < 0.001. Results representative of two independent experiments. Results in (D), (E), and (H) representative of three independent experiments. |
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Reprinted from Immunity, 47(3), Cambier, C.J., O'Leary, S.M., O'Sullivan, M.P., Keane, J., Ramakrishnan, L., Phenolic Glycolipid Facilitates Mycobacterial Escape from Microbicidal Tissue-Resident Macrophages, 552-565.e4, Copyright (2017) with permission from Elsevier. Full text @ Immunity