ZFIN ID: ZDB-FIG-171024-15
Cambier et al., 2017 - Phenolic Glycolipid Facilitates Mycobacterial Escape from Microbicidal Tissue-Resident Macrophages. Immunity   47(3):552-565.e4 Full text @ Immunity
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 2

Mycobacteria Mediate CCR2-Dependent Monocyte Recruitment by Actively Inducing CCL2 in Resident Macrophages

(A) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type or myeloid cell-deficient fish with 250–300 wild-type Mm.

(B–D) In situ hybridizations against zebrafish ccl2 mRNA following hindbrain ventricle infections with vehicle (bacterial media) (B), 80 wild-type Mm (C), 80 Mm - PGL− (D). Black arrows, ccl2 mRNA-positive phagocytes; white arrows ccl2 mRNA-negative phagocytes. Scale bar, 50μm. Results representative of three independent experiments.

(E) Mean brain resident macrophage numbers of csfr1+/− and csfr1−/− zebrafish at 2dpf. Results representative of two independent experiments.

(F) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of csfr1+/− or csfr1−/− fish after infection with 80 wild-type Mm.

(G) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type fish with 250–300 live or heat-killed wild-type Mm.

(H) Mean resident macrophage and monocyte recruitment from 5 to 120 mpi in the HBV of wild-type fish after infection with 80 live or heat-killed (HK) wild-type Mm.

(I) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of csfr1+/− or csfr1−/− fish after infection with 80 Mm - PDIM.

(J) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of wild-type fish after infection with 80 live or heat-killed (HK) Mm - PDIM.

(K) Mean resident macrophage and monocyte recruitment from 5 to 120 mpi in the HBV of wild-type fish after infection with 80 wild-type or PDIM Mm.

Results in (F) and (H) through (K) representative of at least three independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccl38a.5 AB bacterial treatment by injection: Mycobacterium marinum Long-pec fourth ventricle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
csf1raj4blue/j4blue(AB) standard conditions Long-pec brain macrophage decreased amount, abnormal
csf1raj4blue/j4blue(AB) bacterial treatment by injection: Mycobacterium marinum Long-pec brain immune response in brain or nervous system decreased efficacy, abnormal
Long-pec fourth ventricle macrophage decreased amount, abnormal
Long-pec fourth ventricle monocyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Immunity for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Immunity, 47(3), Cambier, C.J., O'Leary, S.M., O'Sullivan, M.P., Keane, J., Ramakrishnan, L., Phenolic Glycolipid Facilitates Mycobacterial Escape from Microbicidal Tissue-Resident Macrophages, 552-565.e4, Copyright (2017) with permission from Elsevier. Full text @ Immunity