IMAGE

Fig. 2

ID
ZDB-IMAGE-171024-2
Genes
Source
Figures for Cambier et al., 2017
Image
Figure Caption

Fig. 2

Mycobacteria Mediate CCR2-Dependent Monocyte Recruitment by Actively Inducing CCL2 in Resident Macrophages

(A) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type or myeloid cell-deficient fish with 250–300 wild-type Mm.

(B–D) In situ hybridizations against zebrafish ccl2 mRNA following hindbrain ventricle infections with vehicle (bacterial media) (B), 80 wild-type Mm (C), 80 Mm - PGL− (D). Black arrows, ccl2 mRNA-positive phagocytes; white arrows ccl2 mRNA-negative phagocytes. Scale bar, 50μm. Results representative of three independent experiments.

(E) Mean brain resident macrophage numbers of csfr1+/− and csfr1−/− zebrafish at 2dpf. Results representative of two independent experiments.

(F) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of csfr1+/− or csfr1−/− fish after infection with 80 wild-type Mm.

(G) ccl2 messenger RNA levels (mean ± SEM of three biological replicates) induced at 3 hr after caudal vein infection of 2 dpf wild-type fish with 250–300 live or heat-killed wild-type Mm.

(H) Mean resident macrophage and monocyte recruitment from 5 to 120 mpi in the HBV of wild-type fish after infection with 80 live or heat-killed (HK) wild-type Mm.

(I) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of csfr1+/− or csfr1−/− fish after infection with 80 Mm - PDIM.

(J) Mean resident macrophage and monocyte recruitment from 5 to 150 mpi in the HBV of wild-type fish after infection with 80 live or heat-killed (HK) Mm - PDIM.

(K) Mean resident macrophage and monocyte recruitment from 5 to 120 mpi in the HBV of wild-type fish after infection with 80 wild-type or PDIM Mm.

Results in (F) and (H) through (K) representative of at least three independent experiments.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Immunity, 47(3), Cambier, C.J., O'Leary, S.M., O'Sullivan, M.P., Keane, J., Ramakrishnan, L., Phenolic Glycolipid Facilitates Mycobacterial Escape from Microbicidal Tissue-Resident Macrophages, 552-565.e4, Copyright (2017) with permission from Elsevier. Full text @ Immunity