FIGURE

Fig. 5

ID

ZDB-FIG-170908-30

Publication

Brunt et al., 2017 - Wnt signalling controls the response to mechanical loading during Zebrafish joint development

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Fig. 5

Wnt manipulation affects cell proliferation and cell morphology at the jaw joint, revealed using the Zebrabow transgenic line. (A-A″): Tg(ubi:Zebrabow) and Tg(Sox10:cre) transgenic lines generate multiple colours of fluorescence in zebrafish cartilage, including at the region of interest at the retroarticular process (RAP) (white dotted line). Cell outlines were created at the RAP: (A′) RAP cell outlines overlaid with confocal image; (A″) cell outlines. (B,C,D) 3 and 5 dpf Tg(ubi:Zebrabow) and Tg(Sox10:cre) control (B), IWR-1-treated (C) and Wnt16 MO-injected zebrafish (D) (n=10, 11 and 20 joints). The RAP of the MC jaw joint is shown. Asterisks of different colours mark cells at 3 and 5 dpf (indicating re-identification and cell division events). (B′,C′,D′) Outlines of individual cells in the RAP of the MC jaw joint, identified in control (B′), IWR-1-treated (C′) and Wnt16 MO-injected (D′) Tg(ubi:Zebrabow) and Tg(Sox10:cre) transgenic zebrafish. Outline colour of individual cells in B′,C′,D′ matches the asterisk colours in B,C,D.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	a131Tg; ba74Tg a131Tg; ba74Tg + MO1-wnt16
Condition:	chemical treatment by environment: IWR-1-endo
Knockdown Reagent:	MO1-wnt16
Observed In:	retroarticular retroarticular cell population proliferation
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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