FIGURE

Fig. 8

ID

ZDB-FIG-170907-17

Publication

Pfefferli et al., 2017 - The careg element reveals a common regulation of regeneration in the zebrafish myocardium and fin

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Fig. 8

Monitoring the primordial CM layer between the compact and trabecular myocardium.

(a) Longitudinal sections of careg:dmKO2;cmlc2:EGFP transgenic adult hearts labelled with antibodies against KO2 (red) and Tropomyosin (blue, white). V, ventricle; A, atrium; BA, bulbus arteriosus. (b) careg:dmKO2 expression is maintained during adulthood in a single layer of thin subcortical CMs (arrowheads) in the junctional zone (JZ) between the compact/cortical (CoM) and the trabecular myocardium (TrM). N=4. (c) A higher magnification of the adult ventricle wall of double transgenic fish careg:dmKO2; careg:EGFP displays an overlapping expression of both markers in subcortical myocytes (arrowheads). N=4. (d) A magnified adult ventricle wall of careg:dmKO2 transgenic fish displays an abundant expression of N-cadherin (green, white) on the surface of the junctional CMs on the side facing the trabecular myocardium. N=4. (e) A fragment of the adult ventricle wall of careg:dmKO2;wt1a-6.8kb:GFP transgenic fish displays the separation of compact myocardium and subcortical careg:dmKO2⁺ layer by wt1a-6.8kb:GFP-labelled fibroblasts of the junctional zone. N=4. (f–i) At 90 d.p.ci., transversal heart sections of careg:EGFP hearts display a gap in the primordial layer within the regenerated myocardium. The extent of the gap is indicated with arrowheads. N=4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.