FIGURE

Fig. S7

ID

ZDB-FIG-170829-29

Publication

F�rster et al., 2017 - An optogenetic toolbox for unbiased discovery of functionally connected cells in neural circuits

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Fig. S7

Accuracy of registration and addition of anatomical reference patterns. (A) Three 5 dpf Optobow-nPA-expressing larvae stained for HuC have been registered into one reference brain. Note the accuracy of registration at different z levels (labeled as distance from dorsal skin in ?m). Scale bar, 30 ?m. (B) Two 5 dpf larvae expressing GCaMP6s under control of isl2b:Gal4 (stained for GFP) were co-registered into the reference brain. Scale bar, 20 ?m. (C) A closeup of the tectal neuropil shows the different innervation strata of RGC axons. SO, stratum opticum; SFGS, stratum fibrosum et griseum superficiale; SGC, stratum griseum centrale; SAC/SPV, stratum album centrale/stratum periventriculare. Scale bar, 20 ?m. (D) Intensity profiles through the boxed region in (C) show the alignment accuracy of RGC innervation strata. (E) Examples of three co-registered cell pairs and their dendritic and axonal arborizations in specific tectal layers. Insets taken from Figure 8. Scale bar, 20 ?m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.