FIGURE

Fig. 7

ID

ZDB-FIG-170822-7

Publication

Ke et al., 2017 - G?1 is required for neutrophil migration in zebrafish

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Fig. 7

G?1 signaling regulates F-actin dynamics in neutrophils. Snapshots from confocal time-lapse imaging (Supplementary Movies 7 and 8) of migrating neutrophils expressing Lifeact-GFP in control and gnb1a/b MO1-injected embryos (3 cells in 3 embryos for each group) following wounding. (A-F) Lifeact-GFP. (A?-F?) DsRed. (A??-F??) Ratiometric images of Lifeact-GFP/DsRed. Yellow asterisks: side nearest wound; white arrowheads: sites of Lifeact-GFP enrichment in leading and trailing fronts of neutrophils.

Expression Data

Gene:	GFP
Fish:	e114Tg e114Tg + MO1-gnb1a + MO2-gnb1b + MO4-tp53
Condition:	amputation: caudal fin
Knockdown Reagents:	MO1-gnb1a MO2-gnb1b MO4-tp53
Anatomical Term:	neutrophil
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	e114Tg + MO1-gnb1a + MO2-gnb1b + MO4-tp53
Condition:	amputation: caudal fin
Knockdown Reagents:	MO1-gnb1a MO2-gnb1b MO4-tp53
Observed In:	neutrophil
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 428(1), Ke, W., Ye, D., Mersch, K., Xu, H., Chen, S., Lin, F., G?1 is required for neutrophil migration in zebrafish, 135-147, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.