Fig. 1

Polq Is Required for Survival following Cas9-Induced DNA DSBs

All images are of∼36 hr post-fertilization (hpf) embryos. Additional images are available in Figure S2. Sequences of the three gRNAs used are available in Table S1.

(A) Homozygous MZpolq mutant.

(B) WT embryo injected with Cas9 protein and gRNA for the gria3a gene.

(D) [sic] WT embryo injected with Cas9 protein and gRNA for the camk2g gene.

(D) MZpolq mutant injected with Cas9 protein and gRNA for the gria3a gene.

(E) MZpolq mutant injected with Cas9 protein and gRNA for the camk2g gene.

(F) Survival rates and fraction of deformed embryos observed for MZpolq and wild-type embryos injected with Cas9 protein and gRNAs targeting one of three genes. Embryos were injected at the one-cell stage, and all data were collected at ∼36 hpf. Depictions of the observed deformities are available in Figure 1 and Figures S2A–S2C. The experiment was performed twice, with n = 15–47 for each injection.

See also Figure S2 and Table S1.