PUBLICATION
Polq-Mediated End Joining Is Essential for Surviving DNA Double-Strand Breaks during Early Zebrafish Development
- Authors
- Thyme, S.B., Schier, A.F.
- ID
- ZDB-PUB-160507-11
- Date
- 2016
- Source
- Cell Reports 15(4): 707-714 (Journal)
- Registered Authors
- Schier, Alexander, Thyme, Summer
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 27149851 Full text @ Cell Rep.
Citation
Thyme, S.B., Schier, A.F. (2016) Polq-Mediated End Joining Is Essential for Surviving DNA Double-Strand Breaks during Early Zebrafish Development. Cell Reports. 15(4):707-714.
Abstract
Error-prone repair of DNA double-strand breaks (DSBs) has been postulated to occur through classical non-homologous end joining (NHEJ) in systems ranging from nematode somatic tissues to zebrafish embryos. Contrary to this model, we show that zebrafish embryos mutant for DNA polymerase theta (Polq), a critical component of alternative end joining (alt-EJ), cannot repair DSBs induced by CRISPR/Cas9 or ionizing radiation. In the absence of DSBs, polq mutants are phenotypically normal, but they do not survive mutagenesis and display dramatic differences in the mutation profiles compared with the wild-type. These results show that alt-EJ repair is essential and dominant during the early development of a vertebrate.
Errata / Notes
Correction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping