Fig. 1 S1

Effectiveness of sec14l3 MOs and generation of sec14l3 mutants.

(A) Target sites of sec14l3 MOs. 5’ UTR of sec14l3 is shown and the target sites of MOs are outlined with start codon indicated in red.(B) Effectiveness of the sec14l3 MOs. Embryos were injected with 75 pg pSec14l3-5’UTR-GFP reporter plasmid DNA in combination with indicated MOs and fluorescence was checked at late gastrulation stages. Scale bars, 500 μm.(C) Generation of sec14l3 mutants with TALENs. Four different lines were identified and their genotypes are labeled at the right. The ‘N’ in tdN indicates the total number of edited nucleotides. (D) sec14l3 transcript expression in Msec14l3, Zsec14l3 and MZsec14l3 mutant embryos at 2 cell stage. Embryos are shown at lateral views. Scale bars, 100 μm. (E) Morphology of Msec14l3 embryos at 20 hpf, 24 hpf, 48 hpf and 72 hpf. The embryonic body lengths at indicated stages were measured and the statistical analysis is shown at the right. Data are presented as mean ± SEM. n = 10 embryos (see also Figure 1—source data 1, **p<0.01; *p<0.05). Scale bars, 100 μm.