Fig. 2
- ID
- ZDB-FIG-170609-37
- Publication
- Theodore et al., 2017 - Distinct Roles for Matrix Metalloproteinases 2 and 9 in Embryonic Hematopoietic Stem Cell Emergence, Migration, and Niche Colonization
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Inhibition of Mmp2, but Not Mmp9, Function Affects EHT in the VDA (A) Exposure to prinomastat (20 μM) or ARP-101 (10 μM; 12–36 hpf) caused abnormal runx1/cmyb patterning in the VDA, while MMP9-I (5 μM) had no effect. (B) Qualitative phenotypic distribution of embryos from (A) scored with normal or abnormal runx1/cmyb expression (n ≥ 20/condition). (C) In vivo imaging of Flk1:GFP at 36 hpf indicated that MMP inhibitor (12–36 hpf) treatment did not affect physical vasculature structure (n ≥ 5 embryos/condition). (D) FACS analysis of double-positive HSPCs in Tg(kdrl:dsred/cmyb:gfp) embryos showed no difference in HSPCs after MMP inhibitor exposure (12–36 hpf; 5 embryos/sample, ≥3 replicates/condition). (E) MO knockdown of mmp2 or mmp9 phenocopied effects of chemical inhibition on runx1/cmyb WISH at 36 hpf. (F) Phenotypic distribution of embryos from (E) scored for runx1/cmyb expression (as in A) in the AGM (n value as in B). (G) MO knockdown of mmp2 or mmp9 had no impact on Flk1:GFP+ endothelium (n value as in C). (H) FACS analysis of Flk1:dsRed+/cMyb:GFP+ HSPCs showed no significant difference between mmp2 or mmp9 morphants and controls at 36 hpf (n value as in D). Arrowheads mark HSPC clusters. Error bars denote mean ± SD. Scale bars, 100 μm. |
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Stage: | Prim-25 |
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Stage: | Prim-25 |