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Fig. S1
Amino acid sequence alignment of GPX4/Gpx4 and spatiotemporal expression pattern of zebrafish gpx4b. (A) Amino acid sequence alignment of human, mouse, and zebrafish GPX4/Gpx4. Identical amino acids are indicated in black and similar amino acids are indicated in grey. The Sec residue (U) is indicated with an asterisk and highlighted in red. The accession numbers are as follows: human GPX4 NP_002076.2, mouse GPX4 NP_032188.3, zebrafish Gpx4b NP_001025241.2, zebrafish Gpx4a NP_001007283.2. (B) RT-PCR analysis of zebrafish gpx4b mRNA at the indicated embryonic stages. Numbers indicate different developmental stages as hours post fertilization (hpf). β-actin was used as internal control. +, positive control; -, negative control. (C) Whole-mount in situ hybridization analysis of zebrafish gpx4b mRNA at the indicated stages. Panels are dorsal, top, or lateral views with animal pole up or anterior to the left. Scale bars = 200 μm.