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ZFIN ID: ZDB-FIG-170524-4
Thomas-Jinu et al., 2017 - Non-nuclear Pool of Splicing Factor SFPQ Regulates Axonal Transcripts Required for Normal Motor Development. Neuron   94(2):322-336.e5 Full text @ Neuron
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. S1

(Related to Figure 1)

a. Temporal phenotypic progression in sfpq mutant Lateral view of live WT siblings and sfpq mutant with anterior to the left at various developmental stages: 28hpf (n=31), 30hpf (n=47), 38hpf (n=21), 48hpf (n=19), 52hpf (n=41) and 58hpf (n=71), with 25% of the total (n) are homozygous mutants . Scale bar=100μm.

b. Normal overall brain patterning and induction of MHB in sfpq mutant Lateral view of zebrafish brain (a-l) and spinal cord (m,n,q,r) with anterior to the left. Normal induction of MHB (black arrowhead) is observed in sfpq mutants with normal expression of pax2.1 and fgf8 at the MHB at 15ss (n=55; n=62). Expression of fgf8 and foxb1.2 in the MHB (white arrowhead) is greatly reduced in the sfpq mutants ( n=10/40; n=5/21) by end of somitogenesis. No difference in the pattern of netrin1 and lhx5 expression was detected (n=9/36; n=6/25).

c. Wnt signaling in the sfpq mutant The axin2 and topd gfp expression was normal in the spinal cord and brain except for its expression (white arrow) in the hypothalamus of WT siblings that is absent in the sfpq-/- embryos (n=11/42; n=7/35) showing the absence of Wnt activity in the region. Scale bar=100μm.

c. Rescue of mutant phentotpye with sfpq RNA injection Dorsal view (a-d) of zebrafish brain at 30hpf and lateral view (e-h) of zebrafish spinal cord at 32hpf with anterior to the left. The embryos from both the sfpq;Tg(isl1:gfp) incross (n=51) and the sfpq;Tg(mnx1:gfp) incross (n=76) injected with sfpq mRNA appeared almost normal compared to its control WT siblings with the rescue of axonogenesis defect and the kinky tail that is observed in the non-injected sfpq mutants. Scale bar=100μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
axin2 WT standard conditions Prim-5 brain ISH
Prim-5 spinal cord ISH
Prim-5 tail bud ISH
sfpqkg41/kg41 standard conditions Prim-5 brain ISH
Prim-5 spinal cord ISH
Prim-5 tail bud ISH
fgf8a WT standard conditions Prim-15 midbrain hindbrain boundary ISH
sfpqkg41/kg41 standard conditions Prim-15 midbrain hindbrain boundary ISH
foxb1a WT standard conditions Prim-5 midbrain hindbrain boundary ISH
sfpqkg41/kg41 standard conditions Prim-5 midbrain hindbrain boundary ISH
GFP sfpqkg41/kg41 ; w25Tg standard conditions Prim-5 brain ISH
Prim-5 Not Detected hypothalamus ISH
Prim-5 spinal cord ISH
w25Tg standard conditions Prim-5 brain ISH
Prim-5 hypothalamus ISH
Prim-5 spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sfpqkg41/kg41 standard conditions Prim-5 heart looping disrupted, abnormal
Prim-5 midbrain hindbrain boundary foxb1a expression decreased distribution, abnormal
Prim-5 pericardium edematous, abnormal
Prim-5 post-vent region kinked, abnormal
Prim-5 whole organism decreased length, abnormal
Prim-5 whole organism increased curvature, abnormal
Prim-15 heart looping disrupted, abnormal
Prim-15 midbrain hindbrain boundary fgf8a expression decreased distribution, abnormal
Prim-15 pericardium edematous, abnormal
Prim-15 post-vent region kinked, abnormal
Prim-15 whole organism decreased length, abnormal
Prim-15 whole organism increased curvature, abnormal
Prim-25 heart looping disrupted, abnormal
Prim-25 pericardium edematous, abnormal
Prim-25 post-vent region kinked, abnormal
Prim-25 whole organism decreased length, abnormal
Prim-25 whole organism increased curvature, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec pericardium edematous, abnormal
Long-pec post-vent region kinked, abnormal
Long-pec whole organism decreased length, abnormal
Long-pec whole organism increased curvature, abnormal
sfpqkg41/kg41 ; w25Tg standard conditions Prim-5 hypothalamus GFP expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neuron for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neuron