Fig. S1
Cardiac Left-Right Patterning and Sarcomere Assembly are Unaffected in Paxillin MZ Double Mutants. (A) Whole-mount mRNA in situ hybridization for heart-specific cmlc2 expression at the heart-jogging stage (30 hpf) revealed that asymmetric heart patterning is normal in Paxillin MZ double mutant embryos (indicated by direction of arrows). The number of embryos with proper heart-jogging direction over total number examined is indicated for each genotype. (B) Immunostaining for α-Actinin revealed that sarcomere formation is also normal in Paxillin MZ double mutant embryos. The number of embryos with normal Z-disc organization over total number examined is indicated for each genotype. |
Reprinted from Developmental Biology, 425(1), Jacob, A.E., Amack, J.D., Turner, C.E., Paxillin Genes and Actomyosin Contractility Regulate Myotome Morphogenesis in Zebrafish, 70-84, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.