FIGURE

Fig. 2

ID

ZDB-FIG-170523-10

Publication

Jacob et al., 2017 - Paxillin Genes and Actomyosin Contractility Regulate Myotome Morphogenesis in Zebrafish

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Fig. 2

Double Paxillin mutant zebrafish embryos develop gross morphological defects. (A-H) Gross morphology of wild-type (A), homozygous zygotic single pxn mutant (B, C), maternal and zygotic (MZ) single pxn mutant (D, E), zygotic double pxn mutant (F) and MZ double pxn mutant (G, H) embryos at 4 dpf. Defects in double mutants included pericardial edema (arrows in F, G, H), and kinked tail (arrowhead in G). (I) Western blotting of lysates from 4 dpf embryos revealed that double pxn mutants lacked full-length Pxna and Pxnb proteins, including a high molecular weight band possibly corresponding to the Pxnb-ins isoform.

Expression Data

Genes:	pxna pxnb
Antibody:	Ab1-pxn
Fish:	pxna^{sny6500/sny6500} pxnb^{sny7100/sny7100} pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100} pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100} pxna^{sny6500/sny6500}; pxnb^{sny7100/sny7100}
Observed In:	heart post-vent region notochord whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 425(1), Jacob, A.E., Amack, J.D., Turner, C.E., Paxillin Genes and Actomyosin Contractility Regulate Myotome Morphogenesis in Zebrafish, 70-84, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.