FIGURE

Fig. 3

ID

ZDB-FIG-170510-2

Publication

Kasper et al., 2017 - MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

miR-139 Homozygous Mutants Exhibit Heterogeneity in Endothelial Cell Filopodia Number

(A) Lateral view (25×) of Kdrl:GFP⁺ trunk vasculature at the indicated developmental stages. Arrows point to ISVs.

(B) Average ISV length (μm) was determined from confocal projections (n = 9–18). Bar plots show mean ± SEM.

(C) Representative images (40×) of single endothelial cells expressing tol2-Fli1a-H2B-BFP-p2A-EGFP-farnesyl vector in wild-type and miR-139Δ/Δ ISVs. miR-139Δ/Δ images depict endothelial cells with excessive (top) or few (bottom) filopodia. Endothelial cell nuclei are in blue and membranes are in green.

(D) Bar plots show the average of replicate means ± SEM of endothelial cell filopodia number (n = 9 replicates).

(E) Violin plots show filopodia number probability density distributions. Solid lines in box plots depict median values. Phenotypic variability was significantly different from wild-type, ^∗∗∗p = 9.46 × 10⁻⁴ by Levene's test (n = 36 cells).

(F) Bar plots show average replicate SD ± SEM of endothelial cell filopodia number (n = 9 replicates).

Significance calculations were relative to wild-type embryos. n.s., not significant (p > 0.05); ^∗p ≤ 0.05, ^∗∗∗p ≤ 0.001, two-tailed Mann-Whitney U test unless otherwise indicated. Abbreviations as defined in Figure 1. See also Figure S3.

Expression Data

Gene:	GFP
Fish:	la116Tg dre-mir-139^ya302/ya302; la116Tg
Anatomical Terms:	dorsal aorta intersegmental vessel posterior cardinal vein trunk vasculature
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dre-mir-139^ya302/ya302
Observed In:	intersegmental vessel filopodium
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 40, Kasper, D.M., Moro, A., Ristori, E., Narayanan, A., Hill-Teran, G., Fleming, E., Moreno-Mateos, M., Vejnar, C.E., Zhang, J., Lee, D., Gu, M., Gerstein, M., Giraldez, A., Nicoli, S., MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos, 552-565.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell