FIGURE

Fig. 7

ID

ZDB-FIG-170509-9

Publication

Pradhan et al., 2017 - FGF signaling enforces cardiac chamber identity in the developing ventricle

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Late-differentiating cardiomyocytes contribute to the heart after inhibition of FGF signaling. (A-H) Three-dimensional reconstructions (A,E) and single optical sections (B-D,F-H) of live embryos carrying Tg(myl7:egfp) and Tg(myl7:dsredt4); lateral views at 40 hpf, after heat shock at 18 hpf. Owing to the differential protein-folding kinetics of eGFP and dsRed (Lepilina et al., 2006), reporter transgene expression distinguishes early-differentiating (eGFP⁺dsRed⁺) and late-differentiating cardiomyocytes (eGFP⁺dsRed⁻) (de Pater et al., 2009). As in nontransgenic embryos (A-D, n=6), late-differentiating cardiomyocytes (eGFP⁺dsRed⁻, dashed outlines) contribute to the arterial pole (brackets) in Tg(hsp70:dnfgfr1) embryos (E-H; n=8). Scale bar: 30 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development