FIGURE

Fig. 5

ID

ZDB-FIG-170509-7

Publication

Pradhan et al., 2017 - FGF signaling enforces cardiac chamber identity in the developing ventricle

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Fig. 5

Increased colocalization of Vmhc and Amhc in the ventricle when FGF signaling is inhibited. (A-P) Immunofluorescence showing Vmhc (red) and Amhc (green) localization in embryos treated with SU5402 from 18?hpf. Lateral views are three-dimensional reconstructions (A,E,I,M) or magnified single optical sections, showing both channels (B,F,J,N), green only (C,G,K,O) or red only (D,H,L,P). See Fig. S3 for DMSO-treated control embryos. (A-D) At 26?hpf, the SU5402-treated ventricle contains only Vmhc (n=10). (E-H) By 28?hpf, Amhc is evident within some Vmhc⁺ cells, particularly along the inner curvature (arrowheads; n=9). We presume that these cells are early-differentiating cardiomyocytes, as late-differentiating cardiomyocytes normally contribute to the heart after 30?hpf (Lazic and Scott, 2011). (I-L) By 32?hpf, increasing numbers of Amhc⁺Vmhc⁺ cells are visible in the ventricle (arrowheads; n=11). (M-P) By 48?hpf, higher levels of Amhc accumulate in ectopic cells (arrowheads), in concert with diminishing levels of Vmhc (n=9). Scale bars: 50??m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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